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Literature summary for 2.7.2.1 extracted from
- Purification and characterization of acetate kinase from acetate-grown Methanosarcina thermophila. Evidence for regulation of synthesis (1988), J. Biol. Chem., 263, 15444-15448.
General Stability
| General Stability | Organism |
|---|---|
| the enzyme is unaffected by repeated freezing and thawing | Methanosarcina thermophila |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| KCl | activity linearly decreases from 100% (at 0 mM added KCl) to 71% at 500 mM added KCl | Methanosarcina thermophila |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.8 | - |
ATP | 37°C, pH is not specified in the publication | Methanosarcina thermophila | |
| 22 | - |
acetate | 37°C, pH is not specified in the publication | Methanosarcina thermophila | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | the enzyme requires a divalent cations for activity (Mn2+, Mg2+ Co2+ or Ca2+). Cu2+, Ni2+, or Zn2+ resulted in no significanta activity | Methanosarcina thermophila | |
| Co2+ | the enzyme requires a divalent cations for activity (Mn2+, Mg2+ Co2+ or Ca2+). Cu2+, Ni2+, or Zn2+ resulted in no significanta activity | Methanosarcina thermophila | |
| Mg2+ | the enzyme requires a divalent cations for activity (Mn2+, Mg2+ Co2+ or Ca2+). Cu2+, Ni2+, or Zn2+ resulted in no significanta activity | Methanosarcina thermophila | |
| Mn2+ | the enzyme requires a divalent cations for activity (Mn2+, Mg2+ Co2+ or Ca2+). The maximum rate is obtained with Mn2+ | Methanosarcina thermophila | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
2 * 53000, denaturing gel electrophoresis | Methanosarcina thermophila |
| 87000 | - |
non-denaturing, gradient gel electrophoresis | Methanosarcina thermophila |
| 94000 | - |
gel filtration | Methanosarcina thermophila |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + acetate | Methanosarcina thermophila | the enzyme is proposed to function in the initial activation of acetate for conversion to methane and CO2 | ADP + acetyl phosphate | - |
? | |
| ATP + acetate | Methanosarcina thermophila TM-1 | the enzyme is proposed to function in the initial activation of acetate for conversion to methane and CO2 | ADP + acetyl phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanosarcina thermophila | P38502 | - |
- |
| Methanosarcina thermophila TM-1 | P38502 | - |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| the enzyme is stable to oxygen | Methanosarcina thermophila |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanosarcina thermophila |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:acetate-grown cell | - |
Methanosarcina thermophila | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| frozen in liquid nitrogen, the enzyme is stable for at least months | Methanosarcina thermophila |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + acetate | the enzyme is proposed to function in the initial activation of acetate for conversion to methane and CO2 | Methanosarcina thermophila | ADP + acetyl phosphate | - |
? | |
| ATP + acetate | activity in the reverse direction is 2.6fold greater than in the forward direction (acetate kinase activity) | Methanosarcina thermophila | ADP + acetyl phosphate | - |
r | |
| ATP + acetate | the enzyme is proposed to function in the initial activation of acetate for conversion to methane and CO2 | Methanosarcina thermophila TM-1 | ADP + acetyl phosphate | - |
? | |
| ATP + acetate | activity in the reverse direction is 2.6fold greater than in the forward direction (acetate kinase activity) | Methanosarcina thermophila TM-1 | ADP + acetyl phosphate | - |
r | |
| ATP + propionate | the enzyme phosphorylates propionate at 60% of the rate with acetate | Methanosarcina thermophila | ADP + propionyl phosphate | - |
? | |
| CTP + acetate | activity with TP is 53% of the activity with ATP | Methanosarcina thermophila | CDP + acetyl phosphate | - |
? | |
| GTP + acetate | activity with TP is 79% of the activity with ATP | Methanosarcina thermophila | GDP + acetyl phosphate | - |
? | |
| GTP + acetate | activity with TP is 79% of the activity with ATP | Methanosarcina thermophila TM-1 | GDP + acetyl phosphate | - |
? | |
| ITP + acetate | activity with TP is 83% of the activity with ATP | Methanosarcina thermophila | IDP + acetyl phosphate | - |
? | |
| ITP + acetate | activity with TP is 83% of the activity with ATP | Methanosarcina thermophila TM-1 | IDP + acetyl phosphate | - |
? | |
| additional information | the enzyme is unable to use formate | Methanosarcina thermophila | ? | - |
? | |
| additional information | the enzyme is unable to use formate | Methanosarcina thermophila TM-1 | ? | - |
? | |
| TTP + acetate | activity with TP is 106% of the activity with ATP | Methanosarcina thermophila | TDP + acetyl phosphate | - |
? | |
| UTP + acetate | activity with TP is 80% of the activity with ATP | Methanosarcina thermophila | UDP + acetyl phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 53000, denaturing gel electrophoresis | Methanosarcina thermophila |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Methanosarcina thermophila |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
stable for 15 min, rapidly inactivated at higher temperature | Methanosarcina thermophila |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.4 | acetate kinase activity | Methanosarcina thermophila |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | pH 6.0: about 75% of maximal activity, pH 8.5: about 65% of maximal activity, acetate kinase activity | Methanosarcina thermophila |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Methanosarcina thermophila | isoelectric focusing | - |
4.7 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme is proposed to function in the initial activation of acetate for conversion to methane and CO2 | Methanosarcina thermophila |
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